Identification and cultivation of Ganoderma mushroom species in Namibia and the physicochemical properties, phenolics composition and in vitro antioxidant activity of their infusions select="/dri:document/dri:meta/dri:pageMeta/dri:metadata[@element='title']/node()"/>

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dc.contributor.author Hamwenye, Karlin Kundeinge
dc.date.accessioned 2021-06-25T18:44:40Z
dc.date.available 2021-06-25T18:44:40Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/11070/2934
dc.description A thesis submitted in the fulfilment of the requirements for the Degree of Master of Science in Agriculture (Food Science and Technology) en_US
dc.description.abstract Ganoderma is a fungus that grows in natural habitants from different parts of the world. It is a prized mushroom in developed nations especially in Asian countries due to its health promoting properties such as anticancer, antibacterial, anti-inflammatory, antioxidant, anti-diabetic. These health promoting effects are attributed to bioactive compounds such as the phenolics. However, in developing countries such as Namibia, Ganoderma is still an untapped and barely identified resource. The objectives of this study was to identify and cultivate wild Ganoderma species harvested from different host trees in Namibia and to determine their physicochemical properties (moisture, ash, water absorption and water solubility indices). The phenolics composition (total phenolics (TP), total flavonoids (TF), condensed tannins (CT)) and in vitro antioxidant activity (AA) of hot water infusions of the wild and cultivated mushrooms were also determined. Folin-Ciocalteu, Aluminium Chloride, Vanillin-HCl, DPPH assay methods were used to determine TP, TF, CT and AA, respectively. Ganoderma species were identified using DNA based on the sequences in the GenBank with 98-100% similarity. Two new Ganoderma species: Ganoderma enigmaticum and Ganoderma wiireonse were identified for the first time in Namibia. Wild species had 6.12- 11.70% moisture, 1.91- 5.32% ash, 11.55- 24.40 (g of absorbed water/g of dry sample) water absorption index, 3.60- 24.10% water solubility index, 18.37- 44.78 (mg GAE/g of sample) TP, 0.09- 1.67 (mg QE/g of sample) TF, 2.97- 6.37 (mg CAE/g of sample) CT and 40.8- 49.3% AA. Cultivated species had 9.64- 13.45% moisture, 2.34- 6.20% ash, 13.55- 28.30 water absorption index, 6.40- 25.35% water solubility index, 36.70- 52.73 (mg GAE/g of sample) TP, 0.41- 0.86 (mg QE/g of sample) TF, 11.38- 15.29 (mg CAE/g of sample) CT and 53.6- 63.7% AA. Infusions prepared from cultivated Ganoderma species had higher levels of TP, CT and AA than those prepared from wild Ganoderma species suggesting that they can be potential nutraceuticals and source of healthful antioxidants. Further studies should be done on in vitro and in vivo medicinal effects of Ganoderma species in Namibia. en_US
dc.language.iso en en_US
dc.publisher University of Namibia en_US
dc.subject Ganoderma mushroom en_US
dc.subject Physicochemical properties en_US
dc.subject In vitro antioxidant en_US
dc.subject Infusion en_US
dc.title Identification and cultivation of Ganoderma mushroom species in Namibia and the physicochemical properties, phenolics composition and in vitro antioxidant activity of their infusions en_US
dc.type Thesis en_US


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