Zero Emission Research Initiative (ZERI)
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Browsing Zero Emission Research Initiative (ZERI) by Author "Kadhila-Muandingi, Nailoke P."
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Item Minerals and trace elements in domesticated Namibian Ganoderma species(2015) Mhanda, F.N.; Kadhila-Muandingi, Nailoke P.; Ueitele, Isabella S.Edible and medicinal mushrooms are regarded as the ideal health foods. They are well appreciated for their exquisite taste and flavor and are consumed both fresh and in processed forms. Nutritive value of mushrooms is attributed to their high content of essential amino acids, vitamins, minerals and low lipid content. Regular consumption of whole medicinal and edible mushrooms could introduce a functional or medicinal contribution within the individual’s diet. Medicinal mushrooms may prevent or treat "lifestyle-related diseases". The extent of the health beneficial effect will depend on the level, regularity of consumption, the relevance of medicinal mushrooms and concentrates to the particular disease. The minerals and trace elements in domesticated Namibian Ganoderma mushroom species were determined. The results show that fiber content was found highest with 45 g/100 g of dry weight, followed by calcium with 23 g/100 g, carbohydrate with 23 g/100 g, protein with 18.2 g/100 g, iron with 6.41 g/100 g, copper with 4.3 g/100 g while the least were zinc (2.89 g/100 g) and fat (1.9 g/100 g). The presence of these essential nutrients, which are nutritional requirements of human beings, implies that Ganoderma can be a valuable dietary supplement to boost the immune system and improve general health of people. It was therefore concluded that the domesticated Ganoderma has significant amount of nutrients that makes it a good fungus to be used on daily bases for health benefit.Item Molecular phylogeny of Trametes and related genera from northern Namibia(2018) Ueitele, Isabella S.; Chimwamurombe, Percy M.; Kadhila-Muandingi, Nailoke P.Trametes Fr. is widely characterized as a polyporoid cosmopolitan genus which is presented in almost any type of forest environments. It is characterized by a combination of pileate basidiocarp, porous hymenophore, trimitic hyphal system and thin-walled basidiospores which do not react in Melzer’s reagent. Dry polypores were collected from Northern Namibia and identified as Trametes species based on morphology. Molecular analysis of Internal Transcribed Spacer region 1 (ITS 1) and Internal Transcribed Spacer region 2 (ITS 2) of the collected material revealed inconsistency with morphological identification. The phylogenetic tree was reconstructed using the Neighbour Joining method and reliability for internal branches Assessment was done using the ML bootstrapping method with 500 ML bootstrap replicates applied to 44 unpublished sequences and sequences from GenBank database. Only specimens such as D1-D9, D11 and D13 and specimens F1, I2-I4 and K3-K6 were grouped in the trametoid clade together with Trametes species. Furthermore, the position of Trametes trogii (also known as Coriolopsis trogii) was confirmed to be outside the trametoid clade and more closely related to Coriolopsis gallica. The close relationships of Pycnoporus and Trametes were confirmed by grouping of Pycnoporus sanguineus in to trametoid clade. Alignment with GenBank sequences revealed identity to Trametes species with up to 99%. These results suggest that it is better to keep a single generic name of Trametes for the trametoid clade.