Browsing by Author "Kadhila-Muandingi, Nailoke P."

Now showing 1 - 5 of 5
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    Ethnomycology of indigenous trametes mushrooms from northern Namibia
    (University of Namibia, 2017) Ueitele, Isabella S.; Kadhila-Muandingi, Nailoke P.; Chimwamurombe, Percy M.
    To date there is no documented data available on the Namibian indigenous Trametes mushrooms. The objective of this study was therefore to generate information for the first time, on the ethnomycology of Trametes species in Northern Namibia. A questionnaire was used and the data captured were analysed in SPSS. Results show that 83.9% of respondents included in this study knew Trametes mushroom but only 70.4% used it for medicinal purpose. Indigenous Trametes mushrooms in Namibia are mostly used as a tranquiliser to calm bereaved people who are crying hysterically at burials. Other uses include treating cattle from lung disease. These uses for Trametes species have not been reported before in literature.
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    Minerals and trace elements in domesticated Namibian Ganoderma species
    (2015) Mhanda, F.N.; Kadhila-Muandingi, Nailoke P.; Ueitele, Isabella S.
    Edible and medicinal mushrooms are regarded as the ideal health foods. They are well appreciated for their exquisite taste and flavor and are consumed both fresh and in processed forms. Nutritive value of mushrooms is attributed to their high content of essential amino acids, vitamins, minerals and low lipid content. Regular consumption of whole medicinal and edible mushrooms could introduce a functional or medicinal contribution within the individual’s diet. Medicinal mushrooms may prevent or treat "lifestyle-related diseases". The extent of the health beneficial effect will depend on the level, regularity of consumption, the relevance of medicinal mushrooms and concentrates to the particular disease. The minerals and trace elements in domesticated Namibian Ganoderma mushroom species were determined. The results show that fiber content was found highest with 45 g/100 g of dry weight, followed by calcium with 23 g/100 g, carbohydrate with 23 g/100 g, protein with 18.2 g/100 g, iron with 6.41 g/100 g, copper with 4.3 g/100 g while the least were zinc (2.89 g/100 g) and fat (1.9 g/100 g). The presence of these essential nutrients, which are nutritional requirements of human beings, implies that Ganoderma can be a valuable dietary supplement to boost the immune system and improve general health of people. It was therefore concluded that the domesticated Ganoderma has significant amount of nutrients that makes it a good fungus to be used on daily bases for health benefit.
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    Molecular phylogeny of Trametes and related genera from northern Namibia
    (2018) Ueitele, Isabella S.; Chimwamurombe, Percy M.; Kadhila-Muandingi, Nailoke P.
    Trametes Fr. is widely characterized as a polyporoid cosmopolitan genus which is presented in almost any type of forest environments. It is characterized by a combination of pileate basidiocarp, porous hymenophore, trimitic hyphal system and thin-walled basidiospores which do not react in Melzer’s reagent. Dry polypores were collected from Northern Namibia and identified as Trametes species based on morphology. Molecular analysis of Internal Transcribed Spacer region 1 (ITS 1) and Internal Transcribed Spacer region 2 (ITS 2) of the collected material revealed inconsistency with morphological identification. The phylogenetic tree was reconstructed using the Neighbour Joining method and reliability for internal branches Assessment was done using the ML bootstrapping method with 500 ML bootstrap replicates applied to 44 unpublished sequences and sequences from GenBank database. Only specimens such as D1-D9, D11 and D13 and specimens F1, I2-I4 and K3-K6 were grouped in the trametoid clade together with Trametes species. Furthermore, the position of Trametes trogii (also known as Coriolopsis trogii) was confirmed to be outside the trametoid clade and more closely related to Coriolopsis gallica. The close relationships of Pycnoporus and Trametes were confirmed by grouping of Pycnoporus sanguineus in to trametoid clade. Alignment with GenBank sequences revealed identity to Trametes species with up to 99%. These results suggest that it is better to keep a single generic name of Trametes for the trametoid clade.
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    Optimization of indigenous Ganoderma lucidum
    (University of Namibia, 2014) Ueitele, Isabella S.; Chimwamurombe, Percy M.; Kadhila-Muandingi, Nailoke P.
    Ganoderma lucidum is a mushroom which shows antitumor, anti-inflammatory and cytoxic activity and grows prolifically in warm climates on decaying hardwood logs and stumps. An experiment was done at the University of Namibia to cultivate the indigenous G. lucidum; however, compared to results from other countries the mushroom took much longer to grow. The objective of this study was to shorten the cropping cycle of the indigenous G. lucidum under cultivation at the University of Namibia. The indigenous mushroom was cultivated according to the established protocol. A suitable indigenous Ganoderma mushroom was selected to make tissue culture, which was used to make the spawn that was inoculated into the woodchips substrate. New ideas were introduced to induce fast growth and optimum yield. The grains were inoculated with more pieces of tissue culture; the substrate was inoculated with increased layers of spawn and mixed with pearl millet husk instead of wheat bran to accelerate the colonization of the substrates. In addition to the hanging bag method used in the previous study, the buried wood log method was also introduced. Temperature and moisture were closely controlled during the experiment. A major highlight of this study was the significant reduction (p < 0:05) in the time it took for the substrate to reach fruiting stage. The substrate mixed with pearl millet husk was completely colonized by the mycelia, two weeks faster than the substrate that was mixed with wheat bran. Since Pearl millet is readily available in Northern Namibia, individuals and communities can substitute the wheat bran with pearl millet husk, which allows the substrate to be ready for fruiting faster, thus shortening the cropping cycle and also reducing the production cost as they no longer have to purchase wheat bran. The buried wood logs did not yield any fruiting bodies, but the hanging bags did produce G. lucidum. The study was successful in producing fruiting bodies in a shorter period. There was no significant improvement in the yield obtained.