Browsing by Author "Polci, Andrea"

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    Complete coding sequences of lumpy skin disease virus strains isolated from cutaneous lesions in Namibian cattle during 2016 outbreaks
    (2020) Di Felice, Elisabetta; Pinoni, Chiara; Khaiseb, S.; Camma, Cesare; Dondona, Andrea Capobianco; Polci, Andrea; Molini, Umberto; Monaco, Federica
    Between September and October 2016, an outbreak of lumpy skin disease (LSD) was monitored in the Okakarara veterinary district of Namibia. The complete coding sequences were obtained for LSD virus isolates from skin nodules from two symptomatic animals.
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    Genomic diversity of Rift Valley fever strains circulating in Namibia in 2010 and 2011
    (Viruses, 2020-12-16) Cosseddu, Gian Mario; Magwedere, Kudakwashe; Molini, Umberto; Pinoni, Chiara; Khaiseb, Siegfried; Scacchia, Massimo; Marcacci, Maurilia; Capobianco Dondona, Andrea; Valleriani, Fabrizia; Polci, Andrea; Monaco, Federica
    Outbreaks of Rift Valley fever (RVF) occurred in Namibia in 2010 and 2011. Complete genome characterization was obtained from virus isolates collected during disease outbreaks in southern Namibia in 2010 and from wildlife in Etosha National Park in 2011, close to the area where RVF outbreaks occurred in domestic livestock. The virus strains were sequenced using Sanger sequencing (Namibia_2010) or next generation sequencing (Namibia_2011). A sequence-independent, single-primer amplification (SISPA) protocol was used in combination with the Illumina Next 500 sequencer. Phylogenetic analysis of the sequences of the small (S), medium (M), and large (L) genome segments of RVF virus (RVFV) provided evidence that two distinct RVFV strains circulated in the country. The strain collected in Namibia in 2010 is genetically similar to RVFV strains circulating in South Africa in 2009 and 2010, confirming that the outbreaks reported in the southern part of Namibia in 2010 were caused by possible dissemination of the infection from South Africa. Isolates collected in 2011 were close to RVFV isolates from 2010 collected in humans in Sudan and which belong to the large lineage containing RVFV strains that caused an outbreak in 2006–2008 in eastern Africa. This investigation showed that the RVFV strains circulating in Namibia in 2010 and 2011 were from two different introductions and that RVFV has the ability to move across regions. This supports the need for risk-based surveillance and monitoring.