Analytical evaluation of eight-cell cow embryos structure, outwards water and inwards cryo-protectants to ensure successful cryopreservation
| dc.contributor.author | Mausse, Francisco | |
| dc.contributor.author | Bezugly, N. | |
| dc.date.accessioned | 2014-06-30T11:23:56Z | |
| dc.date.available | 2014-06-30T11:23:56Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Modern reproductive techniques use cow embryos at different stages of development. Eight-cell embryos feature a layer comprising seven cells (blastomeres), surrounding the inner cell, making a total of eight. This article analyses outwards water paths and inwards cryo-protectants, within the eight-cell cow embryos and effectiveness of their cryopreservation. Reproductive cycles and superovulation in cows were triggered through reproductive hormones injection. After slaughter, oocytes were harvested and eight-cell embryos were produced in vitro fertilization and through embryo culture. The study included morphological observations to visualize embryo structure, distribution of blastomeres, cell-layers and analytical evaluation as well, to assess distribution of penetrating cryoprotectants within the inner-cell mass. Findings based on the eight-cell embryo structure suggest that the quantity of outwards water and inwards cryo-protectants is symmetrically distributed. Although, the characteristic of water and cryo-protectants movement is expected to be much better in eight-cell embryos, cryo-protectants properties and the modality of cryo-protectants should differ from procedures applied in other class of embryos. Survival rate of cryopreserved eight cell embryos was lower (6.3%) as compared to the results for 7–8 days embryos. Obviously, a successful cryopreservation of eight-cell cow embryos can only be achieved through a combination of both technical and biological parameters. | en_US |
| dc.identifier.citation | Mausse, F., & Bezugly, N. (2013). Analytical evaluation of eight-cell cow embryos structure, outwards water and inwards cryo-protectants to ensure successful cryopreservation. International Science and Technology Journal of Namibia, 2(1), 70-77. | en_US |
| dc.identifier.issn | 20267673 | |
| dc.identifier.uri | http://hdl.handle.net/11070/1123 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Namibia | en_US |
| dc.subject | Water and cryo-protectants | en_US |
| dc.subject | Eight-cell embryos and penetrability | en_US |
| dc.title | Analytical evaluation of eight-cell cow embryos structure, outwards water and inwards cryo-protectants to ensure successful cryopreservation | en_US |
| dc.type | Article | en_US |