FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

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dc.contributor.authorQuan, Jenai
dc.contributor.authorLangelier, Charles
dc.contributor.authorMumbengegwi, Davis R.
dc.date.accessioned2020-02-24T08:54:28Z
dc.date.available2020-02-24T08:54:28Z
dc.date.issued2019
dc.description.abstractThe growing prevalence of deadly microbes with resistance to previously life-saving drug therapies is a dire threat to human health. Detection of low abundance pathogen sequences remains a challenge for metagenomic Next Generation Sequencing (NGS). We introduce FLASH (Finding Low Abundance Sequences by Hybridization), a nextgeneration CRISPR/Cas9 diagnostic method that takes advantage of the efficiency, specificity and flexibility of Cas9 to enrich for a programmed set of sequences. FLASH-NGS achieves up to 5 orders of magnitude of enrichment and sub-attomolar gene detection with minimal background. We provide an open-source software tool (FLASHit) for guide RNA design. Here we applied it to detection of antimicrobial resistance genes in respiratory fluid and dried blood spots, but FLASH-NGS is applicable to all areas that rely on multiplex PCR.en_US
dc.identifier.citationQuan, J., et al. (2019). FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences. Nucleic Acids Research, 47(14), 1-9.en_US
dc.identifier.urihttp://hdl.handle.net/11070/2687
dc.language.isoenen_US
dc.subjectAlison Kuchtaen_US
dc.subjectJoshua Batsonen_US
dc.subjectNoam Teyssieren_US
dc.subjectAmy Lydenen_US
dc.subjectSaharai Calderaen_US
dc.subjectAaron McGeeveren_US
dc.subjectBoris Dimitroven_US
dc.subjectRyan Kingen_US
dc.subjectJordan Wilheimen_US
dc.subjectMaxwell Murphyen_US
dc.subjectLara Pesce Aresen_US
dc.subjectKatherine A. Travisanoen_US
dc.subjectRene Siten_US
dc.subjectRoberto Amatoen_US
dc.subjectJennifer L. Smithen_US
dc.subjectAdam Bennetten_US
dc.subjectRoly Goslingen_US
dc.subjectPeter M. Mouranien_US
dc.subjectCarolyn S. Calfeeen_US
dc.subjectNorma F. Neffen_US
dc.subjectEric D. Chowen_US
dc.subjectPeter S. Kimen_US
dc.subjectBryan Greenhouseen_US
dc.subjectJoseph L. DeRisien_US
dc.subjectEmily D. Crawforden_US
dc.titleFLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequencesen_US
dc.typeArticleen_US
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