Molecular differentiation of rifampicin and isoniazid drug resistant Mycobacterium tuberculosis complex isolated from selected parts of Zambia

dc.contributor.authorKapona, O.
dc.contributor.authorKaimoyo, E.
dc.contributor.authorKatongo, C.
dc.date.accessioned2019-11-01T06:32:00Z
dc.date.available2019-11-01T06:32:00Z
dc.date.issued2019
dc.description.abstractThe most prevalent Mycobacterium species in the Mycobacterium tuberculosis complex and frequency of mutations in the genes conferring resistance to rifampicin and isoniazid in Zambia have until now remained unknown. This study sought to differentiate the isolates of Mycobacterium tuberculosis complex (MTBC) using genetic regions cfp32, RD9 and RD12 and determine the prevalence of resistance-associated mutations in two specific resistance marker genes (rpoB and katG) and the inhA promoter region of M. tuberculosis isolated at the University Teaching Hospital Tuberculosis Laboratory between January 2013 and June 2014. Genomic DNA was extracted from 40 isolates and amplified by multiplex PCR for regions cfp32, RD9 and RD12 and single PCRs for three drugresistance conferring loci (katG, rpoB and inhA). All isolates were positive for the three regions typical of M. tuberculosis. Seventy-five percent had the S315T mutation in katG gene and one had mutations in the inhA promoter and rpoB in addition to the katG 315 mutation. The S450L (48.7%) and H445Y (20.5%) mutations were the most frequently observed mutations in the rpoB gene while rifampicin mono-resistance was observed in 2.6% of the rifampicin-resistant isolates. Molecular diagnostic tests based on detecting these predominant mutations could be useful for the rapid detection of multi drug resistant tuberculosis in Eastern, Lusaka, Western parts of Zambia and TB patients in these regions can be treated with the standard first line tuberculosis drugs.en_US
dc.identifier.citationKapona, O., Kaimoyo, E., & Katongo, C. (2019). Molecular differentiation of rifampicin and isoniazid drug resistant Mycobacterium tuberculosis complex isolated from selected parts of Zambia. International Science and Technology Journal of Namibia, 13, 24-37.en_US
dc.identifier.urihttp://hdl.handle.net/11070/2624
dc.language.isoenen_US
dc.publisherUniversity of Namibiaen_US
dc.subjectTuberculosisen_US
dc.subjectRifampinen_US
dc.subjectIsoniaziden_US
dc.subjectMutationsen_US
dc.titleMolecular differentiation of rifampicin and isoniazid drug resistant Mycobacterium tuberculosis complex isolated from selected parts of Zambiaen_US
dc.typeArticleen_US
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