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14C dating of organic residue and carbonate from stromatolites in Etosha Pan, Namibia: 14C reservoir effect, correction of published ages, and evidence of >8-m-deep lake during the late pleistocene
(2013) Hipondoka, Martin; Brook, George A.; Railsback, Bruce L.; Marais, Eugene
Lacustrine stromatolites are layered accretionary structures formed in shallow water by cyanobacteria. They are a precise indicator of high lake limits and their morphology and structure provide an insight into paleoenvironments of the time. Previous research on lacustrine stromatolites from Etosha Pan in Namibia based on radiocarbon ages of carbonates were close to the limit of the method and did not account for any possible 14C reservoir effect. The ages were used to suggest that the basin was not extensively flooded during the last 40,000 yr. To assess the reservoir effect, the age characteristics of a stromatolite from Poacher’s Point were investigated by 14C dating both carbonate and organic residue from samples at different depths in the deposit. The ~15-cm-diameter stromatolite was separated into 12 zones from the center to the edge and block samples were cut from each zone; the carbonate and residual organic residue were dated separately. The carbonate ages ranged from 34,700 to 24,700 14C yr BP and the organic ages from 15,700 to 2500 14C yr BP. Ages generally increased with increasing distance from the surface of the deposit. We believe that the organic ages are an accurate estimate of the stromatolite’s age, while the much older carbonate ages reflect incorporation of old carbon from limestone bedrock and ancient calcrete introduced by stream and spring flow. Excluding the 2 oldest organic ages (15,700 and 13,600 14C yr BP), which may reflect contamination by older organic material washed into the lake during flooding, a linear regression relationship between carbonate and organic ages indicates that the reservoir effect on carbonate ranges up to ~24,000 14C yr BP but decreases slightly as the true age of the deposit increases. This regression relationship was used to correct 2 finite carbonate ages for stromatolites from Pelican Island obtained in the early 1980s, which together with our new organic age for a stromatolite from Andoni Bay, document a >8-m-deep lake in Etosha Pan during the Late Pleistocene, at and prior to ~34,000–26,000 cal yr BP. The organic carbon ages from the Poacher’s Point stromatolite suggest prolonged lacustrine conditions during the early to middle Holocene (8000–6600 cal yr BP) but not to the extent seen during the Late Pleistocene
A blockchain-based land titles registry in Namibia
(University of Namibia, 2024) Paavo, Johannes Pandeni; Puente, Rodriguez Rafael
Land administration systems in many countries are vulnerable and lack transparency, which leads to fraud, delays, and significant costs, especially in developing countries like Namibia. This study aims to address these issues by proposing a blockchain-based land titles registry to preserve land rights in Namibia, drawing inspiration from successful implementations of countries such as Sweden. The study employs a quantitative methodology, using synthetic data to simulate real-world land transactions and scenarios, resulting in a controlled experimental environment. The research findings reveal significant dissatisfaction among Namibians with the existing land administration system as a result of various challenges encountered during land registration. The study proposes a hybrid blockchain network as a promising solution, balancing flexibility, transparency, and privacy, with the potential to reduce bureaucratic delays and improve accessibility for all stakeholders. This network's cryptographic foundation ensures data integrity and increases transparency in land transactions, which may influence land rights protection and stimulate economic growth. Despite its potential, the literature review identifies several challenges, such as contract legality, co-ownership management, and legal framework alignment, that must be addressed before widespread blockchain implementation can occur. This study however lays the groundwork for future investigations into infrastructure scalability, legal framework integration, infrastructure challenges, user adoption strategies, and interoperability issues. These critical areas of research will help understand blockchain's transformative potential for revolutionizing land administration systems
A comparative analysis of GNSS-inferred precipitable water vapour at the potential sites for the Africa Millimetre Telescope
(Monthly Notices of the Royal Astronomical Society, 2025) Frans, Lott; Backes, Michael; Falcke, Heino; Venturi, Tiziana
The Event Horizon Telescope (EHT) is a network of antennas across the globe currently used to image supermassive black holes (SMBHs) at a frequency of 230 GHz. Since the release of the image of M87 in 2019 and, subsequently, that of Sgr A in 2022 by the EHT collaboration, the focus has shifted to dynamically imaging SMBHs. This has led to a search for potential sites to extend and fill in the gaps within the EHT network. The Gamsberg Mountain and the High-Energy Stereoscopic System (H.E.S.S.) site are both located within the Khomas highlands and have been identified as potential sites for the Africa Millimetre Telescope (AMT). Precipitable water vapour (PWV) in the atmosphere is the main source of opacity and noise from atmospheric emissions when observing at millimetre to sub-millimetre wavelengths. This study aims to establish the PWV content and the atmospheric transmission at 86, 230, and 345 GHz at the AMT potential sites using Global Navigation Satellite System derived PWV data. Results show both sites have potential for observations at 86 and 230 GHz, with 345 GHz possible at the Gamsberg Mountain during winter. The overall median PWV of 14.27 and 9.25 mm was calculated at the H.E.S.S. site and the Gamsberg Mountain, respectively. The EHT window had PWV medians of 16.62 and 11.20 mm at the H.E.S.S. site and Gamsberg Mountain, respectively. Among the two sites, the Gamsberg Mountain had the lowest PWV conditions, therefore making it the most suitable site for the AMT
A geochemical baseline study of giant kelp (macrocystis pyrifera) at the kelp blue farm to assess carbon sequestration potential
(University of Namibia, 2024) Mutjida, Protasius Shikusho
The raised levels of carbon dioxide (CO2) in the earth’s atmosphere have contributed to global climate change, which has adversely affected a wide range of individuals, communities, and organizations. One of the ways to mitigate climate change is to grow a huge amount of kelp forests that can absorb carbon dioxide from the atmosphere and sequester it in the deep ocean. To grow these forests, the right biogeochemical conditions (temperature, nutrients, oxygen, salinity, pH, trace metals and ions) within the ocean is vital to assess the sequestration potential and the impacts on the ocean health. This study aimed at monitoring the biogeochemical changes at the proposed Kelp Blue farm and surrounding areas further away from the farm. This study also aims to create a baseline of the chemical composition of the farm and Lüderitz areas, which has not been done before. Using the results obtained from the study, the potential of the cultivated giant kelp to sequester CO2 will be evaluated. This study measured the geochemical parameters using both in-situ probes and laboratory analyses at 10 different locations in Lüderitz (both offshore and coastal): offshore (within the Kelp Blue Pilot project farm, upstream of the farm in the Dumfudgeon rocks, downstream of the farm at Boot Bay and further south of Lüderitz at the Halifax island) and the coastal areas which are adjacent to the offshore study areas including at coastal site of Guano Bay which is about 17 km and the coastal area of Grossebucht which is about 20 km south the town of Lüderitz as another control site. This study also collected sediment samples at these offshore sites including further north of Walvis Bay at the different latitudinal transects. The sediments were analysed for trace metals by using a portable XRF and for carbon content by LOI (Loss on Ignition) method. Another geochemical analysis done was the titrimetric method to CO2 determination of seawater by using NaOH as a titrant the end points were determined. The concentrations of nutrients (phosphates and nitrites) in seawater samples were determined by adding them to a 10 mL cuvette, which had been zeroed first. A specific nutrient reagent was then introduced, and using ii prescribed sample preparation techniques, a spectrophotometer was employed to measure the concentrations of the nutrients in µg/L and mg/L. The geochemical parameters changed in relation to the seasonality and geographical positioning. In a season when the upwelling system was strong, the concentration of nutrients, acidity and the value of the pH, salinity, other geochemical parameters increased more, compared to other seasons and the further southern sampling sites resembled to that of the Southern Benguela Upwelling System (SBUS). Higher concentrations of phosphates (614 µg/L) were found in areas close to the islands. This is due to the presence of guanos. These remarkable long duration geochemical measuring results shows that the cultivated kelp can co-exist with other natural existing kelp beds in this ecosystem, and they can thrive more predominantly in season of great influx of nutrients during the season of strong winds. With a dynamic Benguela upwelling eastern boundary, these kelp forests have an enormous potential to grow more and will function as carbon absorber and eventually sequester more carbon when cultivated at larger scale. To quantify the carbon sequestration potential of these giant kelps, there are other areas to be investigated such as developing a sediment carbon baseline using isotopic analyses, lipid biomarkers, identifying sediment hotspots where the kelp will be deposited, developing a carbon dioxide removal model and geochemical dispersion models. All these other areas will be developed from the baseline being developed by this study. This study recommends that a continuous and rigorous monitoring of the water and sediment geochemical changes be done as the kelp forest grows and as the scale of operation expands
A geochemical study of gallium, germanium and indium, in sulfide mineralisation of the Kombat, Tsumeb and Khusib springs deposits, Otavi Mountain Land, Namibia
(University of Namibia, 2024) Shalimba, Ester; Wanke, Ansgar
Carbonate-hosted base metal deposits are known to contain significant contents of critical metals such as Ga, Ge, and In. These metals occur mainly as inclusions or incorporated within the crystal lattice of the main sulfide minerals while they form rarely discrete minerals. The aim of this Doctor of Philosophy (PhD) study is to determine the geochemical characteristics of the critical raw metals that are associated with the sulfide mineralisation of the base metal deposits of the Otavi Mountain Land, with the emphasis on the Tsumeb, Kombat and Khusib Springs deposits. Tsumeb mine is an over the decades exploited breccia-pipe like polymetallic deposit, hosted by Neoproterozoic dolomite and limestones in the Otavi Group of the Damara Orogenic Belt. Exploitation took place between 1897 and 1996, with a production of about 30 Mt of ore, yielding 1.7 Mt Cu, 2.8 Mt Pb, 0.9 Mt Zn, and 80 t Ge. Mineralisation at this deposit consists of tennantite and sphalerite as the most abundant ore minerals, associated with galena, pyrite, bornite, chalcocite, digenite, and minor enargite, renierite, germanite, chalcopyrite, and traces of various arsenates. Kombat deposit, which is a stratabound, syntectonic Cu-Pb (Ag) sulfide deposit, is hosted in the dolomite of the Hüttenberg Formation, Otavi Group. Mining of the Kombat deposit is currently ongoing, with a total endowment of approximately 13.6 Mt, of which 12 Mt has been exploited until 2008. The ore mineralisation of the Kombat deposit is quite variable comprising massive, disseminated, veinlet-controlled, and brecciated mineralisation styles with abundant bornite, chalcopyrite, galena, covellite, and chalcocite set into quartz-calcite gangue. Khusib Springs deposit is a high-grade Cu-Pb Zn deposit which is classified as a “Tsumeb-type deposit”, hosted by Neoproterozoic dolomite, limestone and phyllites of the Maieberg Formation, Otavi Group. The main aim of this study is to show the siting of the most important minor and trace elements in the diverse sulfide mineral phases and to highlight the relationships between common trace elements and critical metals within the sulfide mineral phases. Various analytical techniques, including laser ablation inductively coupled plasma spectrometry (LA-ICP MS), electron microprobe analysis (EPMA), inductively coupled plasma mass spectrometry (ICP-MS), and X-ray fluorescence (XRF), were employed to study the bulk ii geochemistry and the siting of the trace elements, for the sole purpose of answering the above-mentioned objectives. Additionally, portable X-ray fluorescence (pXRF) technique was utilized with the aim of evaluating the possible usability of a low-cost analytical technique in exploration for base and critical elements in carbonate-hosted sulfide mineralisations. The Kombat mineralization comprises three mineral assemblages (1) bornite-chalcopyrite-covellite, (2) bornite-galena-chalcocite, and (3) bornite-chalcopyrite, set into dolomite-quartz-calcite gangue, with chalcopyrite identified as the main carrier of Ga, Ge, and In in this deposit. The profound micro-analysis of Tsumeb ore revealed the presence of both Ga and Ge, associated with trace elements (Ag, As, Cd, Co, Cu, Fe, Ga, Ge, In, Ni, Pb, Sb, Zn), in the different sulfides. The most significant carriers of Ge are renierite (av. 8.21 wt.%) and enargite (0.78 wt.%), while Ga is mainly incorporated into renierite (av. 0.12 wt.%) and sphalerite (av. 0.11 wt%). Trace element abundance in sphalerite is characterized by elevated Cd, Ge, and Ga, suggestive of low formation temperatures, which corroborates with earlier research on the formation of Tsumeb ore. Reconnaissance Zn isotope data (-0.19 to 0.14‰) delineate Tsumeb ore as typical Zn-rich massive sulfide ore, with Zn isotope values overlapping with those of the Irish MVT deposits (-0.17 to 1.33‰), while Cu isotopes are comparable to isotopic data of hypogene mineralisation. In Khusib Springs ore, tennantite (Fe, Zn, Ag, Sb) and chalcopyrite (Cu, Pb, Ag) are the most important carriers of trace elements while all other sulfides are characterized by a surprisingly low trace element content. In contrast, Khusib Springs massive ore reveals a wealth of Ag-bearing minerals of the pearceite-polybasite mineral group. Modern portable tools are successfully applied for the analyses of selected elements in various fields. Portable XRF can be used as well for the analyses of selected elements (Cu, Fe, Mn, Pb, Zn) in Kombat-type ore, while these instruments fail for the analyses of elements like Ag, Sr, and, in particular, for the analyses of the target elements Ga, Ge, and In. Therefore, this study gives a detailed overview about the relationship between critical elements Ga, Ge, and In and associated elements in sulfide ores in the carbonate-hosted base metal deposits of the Otavi Mountain Land, while showing at the same time the limitations when access to modern analytical technologies is limited
A statistical analysis of endogeneity and instrumental variables of education on income in Namibia
(University of Namibia, 2024) Nanyemba, Maria K.
Education is a well-known driver of income and a causal factor for economic prosperity and social development in modern society. It provides one with capital such as qualification, knowledge and skillsets relevant to secure stable gainful employment, as well as increase one’s income, improve health conditions and general welfare. However, the measurement of the influence of education most often suffers from endogeneity suspicion due to the possible presence of a correlation between the education variable and the error term. This presents a critical issue when performing a cause-and-effect relationship analysis as neglecting endogeneity can lead to unreliable estimation results even when dealing with large samples because estimators of the model parameters will be inconsistent. To date, most studies that focuses on analysing the effect of education in Namibia have done so with other factors such as fertility and not income, while most of these studies used regression approaches such as the linear regression and logistic regression methods to perform their respective analyses. However, no study has simultaneously tried to account for the endogeneity of both education and income in Namibia. Thus, using the 2015/2016 Namibia Household Income and Expenditure Survey as well as accounting for the possible presence of endogeneity and for non-linear effects of continuous observed confounders, this study aimed at estimating the impact of education on income in Namibia, in addition to exploring different modelling strategies to capture the relationship between income and education, and other socio-economic and socio demographic factors. A total of 495,311 households containing 2,250,122 individuals aged 18 to 60 years who have been to school and have earned salary and/or wage as their source of income ii in the last 12 months of the survey period were considered in this study. Three different modelling strategies were carried out, namely: (i) Basic Ordinary Least Squares (OLS) regression without considering the clustering, stratification and weighting factors, (ii) Basic OLS regression considering the clustering, stratification and weighting factors, and (iii) Instrumental Variables & Two Stage Least Squares (IV-2SLS) regression, while the best fit modelling strategy to use for exploring the relationship between income and education, and other socio-economic and socio-demographic factors was determined using the Durbin and Wu-Hausman test of endogeneity. Results from this study showed that majority (82.1%) of the households in Namibia derived their source of income from salary and/or wage in 2015/2016. The IV-2SLS was identified as the best modelling strategy to use since the Durbin and Wu-Hausman test of endogeneity confirmed the presence of endogeneity (i.e, education must be endogenous), thus, making the OLS strategies unreliable to use. Furthermore, factors such as education, age, sex, marital status, type of work, employment period, total hours worked and transport mode to work had positive and significant influences on income, while factors such as region, dwelling unit type, main material for the wall, household size, and no contribution to investment had a negative and significant influences. However, the type of employer had a mixed effect on income. In addition, the IVs estimators revealed that the average rate of return of schooling was 13% which was higher compared to the OLS estimates of 5%. It is therefore recommended that the Namibian government and policy makers consider boosting more funding into education, especially in communities within the rural and underdeveloped regions still having traditional dwelling households whose structures are made of corrugated iron/zinc sticks, wooden poles, clay and/or cow dung. In addition, as part of their poverty alleviation strategies, the government should iii ensure the distribution of equitable resources, particularly the educational resources, to enhance the development of rural and underdeveloped regions so that they too can benefit from high-quality education opportunities in the country
A study on the medicinal potential of Artemisia afra, a traditionally used herb in Namibia
(University of Namibia, 2024) Farmer, Wennyth Luzaann; Bruwer, Iwanette
The use of plants as medicines to relieve various symptoms, as well as to manage and cure ailments has been recorded since ancient times. Much interest in these ethnomedicinal plants stems from their long-standing use in traditional medicine. They are still regarded as making important contributions to health care even though great advances have been made in modern medicine. Traditionally, Artemisia afra (‘Wilde als’) is used to treat the common cold, flu and coughs, as well as nasal congestion. During the COVID-19 pandemic, communities in Namibia used ‘Wilde als’ as a treatment for the disease. Little is known about the efficacy and safety of ‘Wilde als’ found in Namibia. This study aimed to identify the bioactive classes of compounds for ‘Wilde als’; as well as to determine the antioxidant, anti-inflammatory, and antiviral activities and the cytotoxic properties of the plant. Extracts of ‘Wilde als’ were prepared by aqueous and organic extraction methods, using water and a mixture of methanol and dichloromethane (1:1 v/v). The phytochemical profile was determined using thin-layer chromatography. The total flavonoid content within the extracts was also determined. The albumin denaturation assay was used for determining the in vitro anti-inflammatory response of the extracts of ‘Wilde als’ using egg albumin derived from a fresh hen’s egg as the protein source. The DPPH antioxidant assay was used to determine the radical scavenging activity of the plant extracts using 2,2-diphenyl-1- picryl-hydrazyl-hydrate also known as DPPH, and the MTT cytotoxicity assay was used to determine the cellular reduction of the tetrazolium salt, 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide, also known as MTT. The Vero E6 cell line, a kidney epithelial cell line was used to determine cell proliferation and survival during the MTT assay. The in vitro antiviral effects of the plant extracts against a pseudovirus of SARS-CoV-2 were determined by calculating the reduction of the SAR-CoV-2 in ii the Vero E6 cells. The extracts of the ‘Wilde als’ possessed flavonoids, coumarins, saponins, steroids and terpenoids, with TFC with the highest at 19.89 % for the organic extracts of the twigs. The extracts of A. afra exhibited significant antioxidant activities ranging from 0.1029 to 9.4197 μg/ml with the highest activity observed for the aqueous extract of the leaves. The extracts of A. afra also exhibited significant anti- inflammatory activity. The highest anti-inflammatory activity of the plant extracts was observed for the organic extract from the combination of the leaves and twigs (87.5 % at 100 μg/ml). The plant extracts displayed antiviral potential against the beta-strain of the SARS-CoV 2 virus, with the organic extracts exhibiting higher inhibition of the viral load with EC50 values of 48.84 μg/ml (leaf extracts), 24.65 μg/ml (twig extracts) and 251.67 μg/ml (combination of the leaves and twigs extracts). The results of the MTT cytotoxicity assay indicated that the aqueous extracts showed little to no cytotoxicity; the organic extracts on the other hand showed high cytotoxicity with IC50 values of 134.12 μg/ml (leaves and twigs), followed by 151.53 μg/ml (twigs) and 182.31 μg/ml (leaves). The findings of this study suggest that ‘Wilde als’ has the potential to be used as an antiviral agent with antioxidant and anti-inflammatory activities as the possible mechanisms of action, with low cytotoxic effects for the aqueous extracts. The organic extracts, however, were cytotoxic, but when used in the correct doses, they can have significant health benefits. This study also provides evidence that the use of ‘Wilde als’ an herbal remedy in the south of Namibia is rational and consistent with research from other regions of Africa
Abundance and diversity of microalgae in freshwater eutrophic systems of Namibia and optimization of lipid and biomass production in Nannochloropsis Limnetica
(University of Namibia, 2017) Garus-Oas, Carol H.
Namibia is one of the driest countries in Sub-Saharan Africa, and man-made dams play an important role on salvaging water. These dams are however periodically frequented by microalgae blooms that pose devastating effects on the water quality, making water treatment processes troublesome and cost prohibitive. However, these unicellular organisms can be exploited in various ways especially when cultivated in controlled and closed systems under optimal conditions of light, temperature, pH and nutrient concentrations. Biomass can be optimized to produce proteins that can be a source of fertilizers, while lipids can be extracted to refine into various hydrocarbons such as biodiesel and bioethanol. It is therefore essential to study the dynamics of these microalgae and provide necessary guidelines on how to utilize them. The first objective of the current study was to assess the seasonal relative genera abundance, diversity, richness and eveness of microalgae in three fresh water eutrophic systems in central Namibia. The hypothesis was to seek whether there is no significant difference in the seasonal relative genera abundance of microalgae per dam in three freshwater eutrophic systems of Namibia namely, Goreangab dam, Von Bach dam and Swakoppoort dam. Microalgae were enumerated with the use of a haemocytometer and identified to genus level during the dry and the wet seasons. Environmental parameters such as turbidity, pH, temperature, dissolved oxygen, macronutrients (i.e. phosphates, nitrates and ammonia) and dam water volumes, were also measured during this time to assess how they are correlated to the relative genera abundance per dam. All statistical analyses were performed using the SPSS software package (IBM statistics Version 23) and differences in data were considered significant at p < 0.05. The Shannon-Weiner diversity index was applied to calculate the microalgae diversity per dam. Evenness Index (J’) and Margalef’s Index (d) was used to calculate the species evenness and species richness, respectively per dam. Results revealed that seasonal relative genera abundance was not normally distributed (p = 0.000) per dam. Von Bach dam seasonal relative genera abundance was statistically not significant (p = 1.000). Goreangab dam seasonal relative genera abundance was statistically not significant (p = 0.652) and Swakoppoort dam relative genera abundance was statistically not significant (p = 0.444). Data presentation of relative genera abundance showed Chlorophyceae as the most dominant class followed by Cyanophycea for Swakoppoort and Von Bach dams for both seasons. Chlorophyceae was the most abundant microalgae class in Goreangab dam during the dry season, while Florideophyceae and Cyanophycea were the second most abundant classes during the wet season. Microcystis was the most abundant genus for Swakoppoort and Goreangab dams during dry and wet seasons. However, in Von Bach dam Aulacoseira was the most abundant microalgae. The Shannon-Weiner diversity index showed that Von Bach dam had the highest diversity of 3.21 followed by Goreangab dam (2.32) and the lowest diversity was found in Swakoppoort dam (2.13). Species evenness varied between 0.46 - 0.65 for Swakoppoort and Goreangab dams, but was higher for Von Bach dam (0.72 - 0.97). Species richness calculated with the Margaleff’s index was around 1 for all the dams and throughout all the seasons, except for Swakoppoort dam during the dry season where it was 1.5. According to the statistical analysis performed, all of the environmental parameters measured for all the dams during the dry and wet seasons were not normally distributed (p = 0.000). Correlation tests showed that all the environmental parameters captured during the time for all the dams were not statistically significant (p > 0.05). According to the literature review, the higher relative abundance of the class Chlorophycea observed during the time of sampling for the current study was due to their higher growth rates. The higher relative abundance of the genera Microcystis was due to their physiological advantage (i.e. nitrogen fixing, surface to volume ratios, intracellular gas vesicles for buoyancy and slimy undulation to enable photomovement) over other microalgae identified during the time of sampling. Microcystis was prominent mostly in Goreangab dam which was characterized by optimum combination of environmental factors such temperature, pH levels and high macronutrients when compared to the other dams. The current study recommends assessment of microalgae diversity and abundance over a one year period with sampling at least every second week. It will be essential to also study other macro-and micronutrients, trace elements, chlorophyll a along with their cumulative effects on microalgae composition. Furthermore, the study advises assessment of topography and mechanical extractions for water transfer on microalgae composition. Moreover, a variety of mesh sizes will constitute a more diversified pool of microalgae to study. The second objective of the study was to optimize the neutral intracellular lipid and biomass production in Nannochloropsis limnetica under variant nitrate concentrations in highly controlled conditions in lab-scaled bioreactors over duration of 14 days. The hypothesis was to seek whether there was no significant difference in growth rate, biomass and neutral intracellular lipid productivity of Nannochloropsis limnetica in “normal nitrate” (3.53 M), “nitrate replete” (7.06 M), “moderate nitrate deplete” (1.765 M), and “high nitrate deplete” (0.8825 M) cultivated under the same conditions of light, pH, temperature and CO2 concentration. The parameters measured throughout this process include optical density to measure growth rates, gravimetrical measurements of bio dry mass/biomass (BDM), ion chromatography measurements for ions, pH as well as sterility tests. All statistical analyses were performed using the SPSS software package (IBM statistics Version 23) and differences in data were considered significant at p < 0.05. During the main-cultivation of Nannochloropsis limnetica, growth was observed in a characteristic pattern consisting of lag, exponential, stationary and declining phases. The growth rates across all of the Flasks during the main-cultivation were not normally distributed (p = 0.012). The statistical tests showed that they were statistically significant (p = 0.001). Bio dry mass increased linearly over time for all of the Flasks from 0 day-1 to the highest on 14 day-1 at the end of the experiment. Bio dry mass was normally distributed (p = 0.055). The statistical test showed that it was statistically not significant (p = 0.939). Bio dry mass and growth rates were moderate and positively correlated and statistically not significant (rs (8) = 0.529, p = 0.077). Relative fluorescence data recorded for all the Flasks was the highest only on the first couple of days during the lag phase and decelerated towards the end of the cultivation period. Therefore, there was no lipid accumulation. The relative fluorescence data captured for neutral intracellular lipid analysis showed that during the cultivation process all of the data were not normally distributed (p = 0.000). Statistical analysis showed it to be statistically significant (p = 0.112). Correlation tests performed between BDM and lipids was strong and negatively correlated and statistically significant (rs (8) = -0.765, p = 0.004). The relationship between lipids and growth rates was moderate, negatively correlated and statistically not significant (rs (8) = -0.501, p = 0.087). The highest growth rates were under “moderate nitrate deplete” at 6 days-1, 11.32 hours at 16.11 OD. The highest BDM value (7780 mg/L) was also captured for “moderate nitrate deplete” at 14 days-1, 19.47 hours at 60.27 mg/L nitrate. The highest relative fluorescence (neutral intracellular lipid content) was captured under “nitrate replete” at 2.387 % at 1 day-1, 6.28 hours and 2550.439 mg/L nitrate. The main-cultivation addressed the hypotheses set out. The study was successful in that it exponentially optimized the biomass production of Nannochloropsis limnetica in each of the four Flasks during the main-cultivation. These results suggest elaborate research on the biomass composition for various outputs such as proteins, lipids, carbohydrates, pigments et cetera. It was discovered during the current study that the various nitrate concentrations did not optimize lipid productivity. Therefore, it is proposed that other macronutrients such as phosphate concentrations along with variables such as light intensity and CO2 be manipulated for lipid optimization. Furthermore, in future studies, the Nile red method should be used in conjunction with other conventional gravimetric methods for lipid quantification and validation for the N.limnetica.
Abundance, species composition and diversity of small mammals and the prevalence and intensity of infestation of associated fleas (Siphonaptera) across an altitudinal gradient along the Ugab river, Namibia
(University of Namibia, 2021) Frans, Alma Inga
The objectives of the study were to determine and compare the abundance, species composition and diversity of small mammals and the associated fleas and to determine and compare the prevalence and intensity of infestation of fleas across an altitudinal gradient along the Ugab River, Namibia. Three sampling sites at different altitudes were selected, Outjo (high altitude site) at about 1300 m above sea level (a.s.l.), Vingerklip (middle altitude site) at about 1000 m a.s.l. and Brandberg (low altitude site) at 400 m a.s. l. Small mammals were trapped in January (hot wet season) and May (cold dry season) in 2018. A total of 159 small mammals belonging to seven rodents and two insectivore species were trapped during the entire study. Small mammals trapped included: Namaqua rock mouse (Micaelamys namaquensis), Natal multimammate rat (Mastomys natalensis), Red rock rat (Aethomys chrysophilus), lesser Red musk shrew (Crocidura hirta), pouched mouse (Saccostomus campestris), bushveld gerbil (Gerbiliscus leucogaster), black-tailed tree rat (Thallomys nigricauda), Acacia rat (Thallomys paedulcus) and bushveld elephant shrew (Elephantulus intufi). The abundance, species composition and diversity of small mammal hosts were not significantly different among the sampling sites during the two seasons (hot wet and cold dry seasons). A total of 139 fleas were collected from small mammals during the study. Three flea species were recorded from small mammal hosts during the study, namely: Xenopsylla cheopis, Xenopsylla brasiliensis and Listropsylla dorripae. The overall intensity of flea infestation (median) per host was not significantly different among the three sampling sites during the hot wet and the cold dry season. Overall infestation prevalence of fleas for the three sites during the hot wet season revealed no significant difference. However, the infestation prevalence of fleas of small mammals was significant among the three sites during the cold dry season. Altitude affects vegetation structure and cover, which in turn affect the small mammal and flea communities. Altitude also affects several climatic factors (temperature, rainfall, humidity), which affect small mammal and flea communities.
Abundance, structure and uses of Baobab (Adansonia Digitata L.) populations in omusati region, Namibia
(2015) Munyebvu, Faith
This study sought to determine the biology and the uses of baobab (Adansonia digitata L.) populations in Outapi and Onesi constituencies in Omusati Region, Namibia. As one of the important Non-Timber Forest Products (NTFPs)-providing species of ecological and socio-economic significance, there is need to have a better understanding of the biology and local uses of the species before its full potential and sustainable harvesting is realized. A comparison of densities, distribution patterns, structure, phenology, stem conditions and uses of baobabs between the two constituencies was done. Field data collection was conducted in April 2014. Road transects were used to sample baobabs in the two sites by purposively choosing the next road to gain access to the next focal tree. Circular plots with a radius of 30m from the edge of the canopy of each focal tree were demarcated. In each plot, diameter at breast height (dbh) of adult and sub-adult trees, height of adults, sub-adults and saplings and their stem conditions, number of fruits on each fruiting baobab tree, the Global Positioning System (GPS) waypoints and coordinates and the land-use types where baobabs occurred were recorded. The results revealed that there was no significant difference in the baobab densities (χ2=2, df=1, p>0.05) and median fruit abundance (U=5550.5, p>0.05) between Outapi and Onesi constituencies. The Chi-square tests detected significant differences in the dbh-size classes (χ2=33.038, df=8, p<0.001) and height classes (χ2=16.295, df=4, p<0.05) between the two study sites. The bell-shaped distribution curve in dbh size-classes in the two sites showed poor recruitment. Onesi constituency had 77% damaged stems compared to 50% in Outapi constituency (χ2=22.705, df=2, p<0.001). Onesi villagers make use of the baobab tree more extensively than Outapi residents (χ2=31.022, df=9, p<0.001). Some of the common uses of baobabs in both study sites include human and livestock consumption and treating various ailments. Poor seedling survival resulting primarily from herbivory, human activities and climate variability hampers baobab recruitment. Considering the poor recruitment due to the above factors, the potential for commercialization of baobabs in the region may not be viable. Therefore, active planting in undisturbed areas, protection of seedlings from livestock coupled with community awareness are vital to ensure recruitment so that effective commercialization and subsistence use is realized and sustainable.
Access coverage for remote connectivity framework (ACRCF): Facilitating scholarship awarding in Namibia
(2015) Ndiwakalunga, Immanuel O.
The corporate world was faced with the challenge of community development. As a result, Namibian companies have been offering scholarships to assist students as best as they can. It was however an insurmountable task to assist the populace without clarity of information or background knowledge. Consequently, the selection of students for scholarships was arguably questionable. Some students awarded scholarships were from rural areas and others from urban areas. However, notice had to be taken of those students in remote area schools where connectivity was lacking. In this age of growing technology, solutions to the dilemma of leaving out worthy students for scholarships should be available with the use of Long Term Evolution (LTE) technologies. The LTE powered technologies would provide seamless interlinking of remote students to the scholarship providers. LTE technologies based on the Access Coverage Remote Connectivity Framework (ACRCF) proposed in this study would deal with the high quality content requirements. The technology would provide a feasible service for the provision of improved interview sessions between students and sponsor selection panels. The interview panels would not have to request students to travel long distances to the interview venues, hence saving travel costs and time by ensuring students are interviewed economically. Interviews would be able to take place over Voice over Internet Protocol (VoiP) and other interfaces under the ACRCF infrastructure. The sponsors would be able to access student information readily at their fingertips through the providence of high quality presented student data on a website maintained under the ACRCF infrastructure. Terminals would be placed at the various remote schools to ensure that student data was updated and ready for use as required. These nodes at the various remote schools would hence be updated with all the student records at the specific remote schools to ensure that there was up-to-date data on the systems at the click of a button. A qualitative research approach was undertaken for the purposes of the study to explore the derivations from case studies. Interviews were held with various sponsors and students to confirm the current state of affairs. They were also held to establish the need for the ACRCF infrastructure to link sponsors to the remote students to improve the scholarship selection process. A quantitative research approach was used in the form of questionnaires handed out to various sponsoring institutions for sampling purposes. The data was analysed with use of the SPSS statistical software program. The findings indicated that selections for scholarships had a need to be improved in the area of equitability. Equitable selection would provide equal opportunities for all students whether in resource rich town centres, rural or remote areas.
Acetylcholinesterase inhibition and Cytotoxic activity of extracts and a novel compound isolated from flowers of the wild everlasting (Helichrysum Argyrosphaerum DC)
(University of Namibia, 2024) Mtuleni, Etuhole; Louw, Stefan
Helichrysum argyrosphaerum is a poisonous plant responsible for livestock losses in southern Africa. Helichrysum species primarily affect the central nervous system when ingested by livestock. However, previous attempts to identify the toxins produced by these plants resulted in only tentative identification of some chemical constituents. This study aimed to isolate and characterize compounds from the flowers of H. argyrosphaerum and to determine the in vitro acetylcholinesterase inhibitory activity and cytotoxicity of the methanol (MeOH) and dichloromethane (DCM) flower extracts and isolated compounds. Several flavonoid glycosides (tentatively identified as apigenin-O-glycosides) as well as an unknown chlorogenic acid analogue were isolated from the methanol extract of H. argyrosphaerum flowers using semi- preparative high-performance liquid chromatography (HPLC). The chemical structures of three isolated compounds were elucidated as apigenin, chamaemeloside, and a novel compound, 14-acetyl chamaemeloside, based on their high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance spectroscopy (NMR) data. Interestingly, both the MeOH and DCM H. argyrosphaerum flower extracts as well as 14-acetyl chamaemeloside induced acetylcholinesterase activity. In the cytotoxicity assay, neither the MeOH and DCM extracts nor 14-acetyl- chamaemeloside exhibited toxicity against HeLa cells. This research marks the initial report of acetylcholinesterase activity and cytotoxicity in both H. argyrosphaerum MeOH and DCM flower extracts, as well as the isolated compound, 14-acetyl- chamaemeloside, against HeLa cells. Consequently, these findings will contribute to future toxicological studies and aid in developing preventative agents that can be used in the treatment of animals poisoned by H. argyrosphaerum
Age dating of groundwater in perched aquifers Okongo area, Ohangwena region
(University of Namibia, 2019) Masule, Nicco Libuku
Knowledge of groundwater age / residence time in Okongo perched aquifer is important in understanding key issues in the evolution of groundwater, recharge rates, recharge mechanisms, resource renewability, flow rate and distribution of recharge areas. The build-up in the atmosphere of trace gases such as sulfur hexafluoride (SF6) and tritium (3H) from over a centenary ago offers a convenient way of dating groundwater up to the age of 60 years. These gases are well mixed in the atmosphere; therefore, their input functions help to provide information about groundwater age, distribution and recharge patterns. In this study, sulfur hexafluoride and tritium were used as an age dating tracer for shallow groundwater. The focus of the study was to understand how age dating of groundwater in a perched aquifer system can help to determine recharge dynamics, e.g. recharge rates, processes, and vulnerability of these aquifers to pollution. The study consequently contributes to the knowledge on shallow groundwater in respect to the effectiveness of age dating methods to determine age / residence time of groundwater in perched aquifers. The study’s objective was to determine the residence time, investigate the distribution of recharge and examine the origins of recharge to the perched aquifer using sulfur hexafluoride, tritium and hydrochemistry, stable isotopes (2H and 18O) methods. A total number of 26 groundwater samples were collected during two separate sampling seasons / campaigns namely in October 2016 (for tritium and stable isotopes testing) and April 2017 (sulfur hexafluoride, hydro-chemistry and stable isotope). Samples were then grouped into sub-sets of six samples (18O and 2H) and five sample sets (SF6, 3H) by method of analysis and by the six (6) sampling sites namely Omboloka 1 and 2, Ohameva, Okamanya, Oshanashiwa and Epumbalondjaba. The results on concentration of SF6 in the groundwater samples indicate that recharge to the perched aquifer happened in 2000 for Omboloka 2, with a groundwater age of 17 years; Ohameva in 2010 with an age of 7 years; Okamanya in 2012 with an age of 5 years; and Omboloka 1 and Oshanashiwa in 2016 with an age of 1 year. Epumbalondjaba borehole was not sampled during the sampling campaign due to the site being flooded. Applying a conservative mixing ratio model, the samples were observed to have a higher proportion of young water compared to old water. This proves that the groundwater in the perched aquifer is young with a short residence time. Furthermore, the tritium method used to calculate theoretic/hypothetic activities suggests that groundwaterat Ohameva, Okamanya, Omboloka 1 and Oshanashiwa, is sub-modern. While results for Epumbalondjaba and Omboloka 2 portray a mix of both sub-modern and modern water. The ionic composition of groundwater in the perched aquifer presents three groups of water types characterised by mixed zone water, Na+-K+-HCO3 and Ca2+-Mg2+-HCO3. The perched aquifer system is dominated by the Ca2+-Mg2+-HCO3 water type which is a result of the dissolution of calcrete and the formation of carbonate-rich rocks derived from recent recharge with short residence times. Stable isotopes show that groundwater in perched aquifer systems is enriched in heavy isotopes and has been subjected to evaporation, which is caused by the prevailing climatic and hydrological conditions in the area. This shows that groundwater recharge to the perched aquifer originate from the local catchment systems. Therefore, it is recommended that thetrace gases (sulfur hexafluoride and tritium) methods should be applied to a wide range of groundwater in the perched aquifers of the Okongo area in order to understand recharge areas better. It is necessary to further investigate the groundwater chemistry and isotopic composition of perched groundwater aquifer in more detail. It is further recommended that a long-term monitoring programme consisting of sampling stations for water levels, water quality and stable isotopes is established.
Age determination and growth rate of the northern Benguela saddine (Sardinops sagax)
(2015) Malakia, Maria
The main objective of this study was to determine sardine age by counting rings on otoliths and to analyse the length, age and growth through regression and Von Bertalanffy Growth models. Otoliths amounting to 826 pairs were collected from the annual sardine research survey of the Ministry of Fisheries and Marine Resources in October 2013. A total of 713 sardine otoliths were mounted on black Perspex plates and aged by means of counting rings using a Zeiss microscope at a magnification of 22x. A micrometer (4 epu = 1 mm) was used to measure the radius and distance of rings from the nucleus on each otolith. In total, six age groups were represented in the sample, including, zero, one year, two years, three years, four years and five year age groups. The one year old age class was the dominant age group. Mean lengths at age were determined for each age group and were as follows: Zero age group had a mean length of 13.8 cm, one year olds were 16.8 cm, two year olds were 19.6 cm, three year olds were 21.4 cm, four year olds 22.4 cm and mean length for the five year age group was 23.4 cm. All these lengths were significantly different from each other, except the four and five year age groups. Fish length and otolith radius relationship was strong (r = 0.75, p < 0.05). The estimated parameters for the Von Bertalanffy Growth model were: = 26.6 cm, K = 0.28, and t0 = 2.69. Growth rate of females (K = 0.2256) was lower than males (K = 0.3188).
Aloe Zebrina Baker
(2010) Ndeunyema, Nghitoolwa E.
An interactive near real-time early warning agricultural system for northern farmers in Namibia
(University of Namibia, 2024) Kamati, Willbard K.; Hashiyala, Valerianus
Preparedness is a vital aspect of disaster mitigation, management, and recovery, especially in agriculture. However, it is quite challenging to prepare for unforeseeable disasters without adequate information on the nature of the disasters, their probable start, and their cessation. In the absence of sufficient disaster predictions and early notices, a community’s readiness is at its lowest. This makes that community extremely vulnerable to the losses associated with the disasters. Meanwhile, agriculture is one of the sectors that has been exposed to different inevitable climatic threats over the years in Namibia. Inconsistencies in broadcasting weather information and early disaster warnings are some of the factors that hinder farmers’ preparedness and mitigation of climatic disasters. Therefore, this study intended to explore the development and use of an early warning agricultural system to contribute to resilience and preparedness for hazards and threats by generating comprehensive and timely warnings for farmers in northern Namibia. The study started with a review of existing early warning agricultural systems in the northern regions of Namibia. Subsequently, a mixed research method was employed to conduct a survey in Outapi and Anamulenge constituencies to get 15 commercial and 73 communal farmers’ past experiences with disasters, information sharing, their expectations, and readiness to adopt the system in mitigating the impact of disasters. Lastly, area-specific historic and near real-time data streams from Namibia Meteorological Service were obtained, and a near real-time interactive early warning agricultural system was ultimately developed using the prototype software development methodology. The study revealed that the use of early warning agricultural systems among Namibian farmers in mitigating agricultural losses is open for exploration, with a high possibility of feasibility to a portion of farmers that has technical resources and skills to adopt the system. However, it may come with limitations to the other portion of farmers with limited access to resources and skills
Analysis of antibacterial activity of Terminalia Sericea and Combretum Imberbe two combretaceae species from Namibia
(University of Namibia, 2019) Mapiye, Samson
The study was conducted to investigate the antibacterial activities of stem and leaves from Terminalia sericea and the live and dead bark from Combretum imberbe. The aqueous and organic crude extracts of the two plants were tested on Gram-negative bacteria Escherichia coli, Shigella sonnei, Serratia marcescens, Helicobacter pylori and Gram-positive bacteria Enterococcus faecalis, Staphylococcus aureus and Clostridium perfringens using the disk diffusion method. All the crude extracts exhibited activity against the tested bacteria species except for extracts from C. imberbe (dead bark). Organic extracts had high activity with minimum inhibitory concentration (MIC) values of 0.1mg/ml for all plant parts against S. aureus , S. marcescens, and H. pylori whilst the aqueous extracts from T. sericea (stem and leaves) and C. imberbe (live bark) had MIC values of 1mg/ml all against S. marcescens and H. pylori. Fractions from the two plants were obtained by vacuum liquid chromatography (VLC) using different solvents with increasing polarity. The organic extract fractions from both plants were observed to have good activity against two of the seven bacterial species that were tested. The dead bark plant extracts from C. imberbe had MIC value of 10mg/ml with 100% Ethyl acetate extracts against the bacteria H. pylori. T. sericea leaf extracts had MIC value of 0.01mg/ml against H. pylori in 100% MeOH fractions whilst the stem extracts had activity at 0.01mg/ml against E. faecalis in 100% Acetone fractions. The results indicate that the two plants have good antibacterial activity hence further research is needed to isolate and identify active compounds which can be developed into effective drugs.
An analysis of antibacterialactivity of Terminalia Sericea and Combretum Imerbe two comretaceae species from Namibia
(University of Namibia, 2019) Samson, Mapiye
The study was conducted to investigate the antibacterial activities of stem and leaves from Terminalia sericea and the live and dead bark from Combretum imberbe. The aqueous and organic crude extracts of the two plants were tested on Gram-negative bacteria Escherichia coli, Shigella sonnei, Serratia marcescens, Helicobacter pylori and Gram-positive bacteria Enterococcus faecalis, Staphylococcus aureus and Clostridium perfringens using the disk diffusion method. All the crude extracts exhibited activity against the tested bacteria species except for extracts from C. imberbe (dead bark). Organic extracts had high activity with minimum inhibitory concentration (MIC) values of 0.1mg/ml for all plant parts against S. aureus , S. marcescens, and H. pylori whilst the aqueous extracts from T. sericea (stem and leaves) and C. imberbe (live bark) had MIC values of 1mg/ml all against S. marcescens and H. pylori. Fractions from the two plants were obtained by vacuum liquid chromatography (VLC) using different solvents with increasing polarity. The organic extract fractions from both plants were observed to have good activity against two of the seven bacterial species that were tested. The dead bark plant extracts from C. imberbe had MIC value of 10mg/ml with 100% Ethyl acetate extracts against the bacteria H. pylori. T. sericea leaf extracts had MIC value of 0.01mg/ml against H. pylori in 100% MeOH fractions whilst the stem extracts had activity at 0.01mg/ml against E. faecalis in 100% Acetone fractions. The results indicate that the two plants have good antibacterial activity hence further research is needed to isolate and identify active compounds which can be developed into effective drugs.
Analysis of causes and risk factors associated with maternal deaths in Namibia
(2015) Mulama, Lihongeni N.
Maternal mortality is a major health concern in Namibia as it is the case in most countries. The rate at which women are dying due to pregnancy related causes is high and the cause as well as risk factors are not well explored. The main objective of this research was to identify the causes and risk factors associated with maternal mortality in Namibia and thus the study described the socio-demographic characteristics and three delays that lead to maternal deaths in Namibia. The paper also aims to propose strategies for midwifery practice, in order to prevent maternal deaths in future. Data on health facility based review of maternal records from 13 regions in the country during the period of 2008-2012 was used. This was then complemented by the 2011 Namibia Population and Housing census data. For the health facility data, a case control study design was applied, with a total number of 154 maternal deaths included in the study as cases and 770 women of reproductive ages who fell pregnant during the study period and survived as controls. A logistic regression model was used to assess the risk factors associated with maternal deaths. For the Census data, measures of maternal mortality, such as maternal mortality ratio, maternal mortality rate, lifetime risk of maternal death, and proportion of maternal deaths among women of reproductive age were estimated. Based on the health facility data, of the 154 maternal deaths reviewed, 58.4% were from direct maternal deaths and 41.6% were from indirect maternal deaths. Haemorrhage (37.8%), eclampsia (24.4%) and puerperal sepsis (23.3%) were the leading causes for direct maternal deaths. About 65% of the haemorrhage cases; 64% of the eclampsia cases and 53% of the puerperal sepsis occurred to women who lived in rural areas. The predominant recognizable indirect causes were HIV (45.3%); pneumonia (23.4%) and tuberculosis (17.2%). Maternal deaths are less likely to occur to women living together with their partner than those who are not living together (OR = 0.53). Most women experienced first (51.3%) and third delays (51.9%). Only few women experienced a second delay (12.9%). However it is important to note that some women experienced more than one delay and in some cases all delays were experienced. Using the 2011 Census data, maternal mortality ratio was 547 deaths per 100 000 live births. The highest ratio of 1954 deaths per 100,000 live births is recorded for women in the age group of 45-49 years. Maternal Mortality Ratio is also observed to be high among the teenage mothers between the age group of 15-19 years with an estimate of 421 deaths per 100,000 live births as compared to 318 deaths per 100,000 live births estimated for women aged 20-24 This analysis reinforced previous findings pointing to the fact that haemorrhage and eclampsia are the leading causes of maternal mortality in Namibia and other developing countries. This indicates the need for better obstetric care, particularly for women over thirty years of age.
Analysis of food safety and food handling knowledge among street food vendors in selected local food markets in Windhoek
(University of Namibia, 2022) Ngalangi, Paulus
Contaminated food leads to foodborne disease outbreaks (FDO) that cause millions of deaths among people every year. In Namibia, many low and middle-income consumers use street-vended foods (SVF) with compromised quality of raw materials, degraded handling and storage conditions that do not meet food safety standards. Therefore, this study was conducted by means of a descriptive, cross-sectional study of six Windhoek locations of Okuryangava, Katutura, Klein Windhoek, Kleine Kuppe, Prosperita, and Otjomuise. Food samples were purchased for laboratory analysis to determine pH, moisture and total plate count (TPC). Systems-Theoretic Accident Models and Processes (STAMP) was adopted, informed by data gathered based on event chain models drawn from street food handlers (SFH). Food-handlers were knowledgeable about the need for hygienic practices such as hand washing (94.6%), and cleaning of utensils (83.5%). Knowledge on food pathogens revealed that 72.1% did not know food pathogens such as Salmonella, Listeria, Campylobacter, and E. coli, meanwhile, only 28.4% did not know that parasites, fungi, and viruses act as agents to cause food poisoning. There is a none-consistent pattern of SFH knowledge with regard to hygienic practices during food handling and processing. pH values were lower (3.21±0.29) for liquid beverage than all solid foods that ranged from 6.81±0.10 to 7.54±0.16. The moisture percentage was higher (90.97±0.85) for liquid beverage than all solid foods that ranged from 37.66±1.20 to 63.28±0.89. High TPC of 5.28±0.49 Log CFU/g was detected in matangara, followed by sausage (5.06±0.99), kapana (3.07±0.47), and oshifima (2.66±1.99). STAMP with ready to use scenarios was introduced to deal with accidents that lead to FDO. Moreover, an assessment of the potency and appropriateness of food preservation methods and preservatives are ii recommended. A robust food safety regulatory regime, including a strong component of food safety training for contamination prevention should be pursued and is recommended for SVF handlers

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