Molecular differentiation of rifampicin and isoniazid drug resistant Mycobacterium tuberculosis complex isolated from selected parts of Zambia
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Date
2019
Authors
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Publisher
University of Namibia
Abstract
The most prevalent Mycobacterium species in the Mycobacterium tuberculosis complex and frequency of mutations in the genes conferring resistance to rifampicin and isoniazid in Zambia have
until now remained unknown. This study sought to differentiate the isolates of Mycobacterium tuberculosis complex (MTBC) using genetic regions cfp32, RD9 and RD12 and determine the prevalence
of resistance-associated mutations in two specific resistance marker genes (rpoB and katG) and the inhA promoter region of M. tuberculosis isolated at the University Teaching Hospital Tuberculosis
Laboratory between January 2013 and June 2014. Genomic DNA was extracted from 40 isolates and amplified by multiplex PCR for regions cfp32, RD9 and RD12 and single PCRs for three drugresistance
conferring loci (katG, rpoB and inhA). All isolates were positive for the three regions typical of M. tuberculosis. Seventy-five percent had the S315T mutation in katG gene and one had mutations in the inhA promoter and rpoB in addition to the katG 315 mutation. The S450L
(48.7%) and H445Y (20.5%) mutations were the most frequently observed mutations in the rpoB gene while rifampicin mono-resistance was observed in 2.6% of the rifampicin-resistant isolates.
Molecular diagnostic tests based on detecting these predominant mutations could be useful for the rapid detection of multi drug resistant tuberculosis in Eastern, Lusaka, Western parts of Zambia and TB patients in these regions can be treated with the standard first line tuberculosis drugs.
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Keywords
Tuberculosis, Rifampin, Isoniazid, Mutations
Citation
Kapona, O., Kaimoyo, E., & Katongo, C. (2019). Molecular differentiation of rifampicin and isoniazid drug resistant Mycobacterium tuberculosis complex isolated from selected parts of Zambia. International Science and Technology Journal of Namibia, 13, 24-37.