Evaluation of antimalarial properties of indigenous plants used by traditional healers in Namibia select="/dri:document/dri:meta/dri:pageMeta/dri:metadata[@element='title']/node()"/>

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dc.contributor.advisor en_US
dc.contributor.advisor en_US
dc.contributor.author Du Preez, Iwanette C. en_US
dc.date.accessioned 2014-02-07T14:08:22Z
dc.date.available 2014-02-07T14:08:22Z
dc.date.issued 2012 en_US
dc.identifier.uri http://hdl.handle.net/11070/560
dc.description A thesis submitted in partial fulfilment of the requirements for the Degree of Master of Science en_US
dc.description.abstract en_US
dc.description.abstract Malaria is on the decline in Namibia and the country is moving towards pre-elimination of the disease. However, some communities preferring traditional medicines and not accepting allopathic medicine may prevent elimination. Ethnomedicines need to be integrated into mainstream malaria case management to achieve malaria elimination by 2020. To do so, they need to be documented and validated to allow their safe and effective use. Seven indigenous plants were investigated for antiplasmodia properties on the basis of their indigenous uses to treat malaria-like ailments. Extracts of Vahlia capensis, Nicolasia costata, Rhigozum brevispinosum, Dicerocarym eriocarpum, Senna occidentalis, Lophiocarpus sp. and Crotalaria flavicarinata were prepared using aqueous and organic solvents. Phytochemical screening was performed to detect the presence of selected antiplasmodial compounds. Growth inhibition studies using cellular infection models of Plasmodium falciparum 3D7 and D10 were carried out to determine anti-plasmodial effects of extracts from Vahlia capensis, Nicolasia costata and Dicerocarym eriocarpum. Mechanistic studies were also conducted to determine the mode of action of the three plants. Phytochemical screening revealed the presence of alkaloids, anthraquinones, flavonoids, terpenoids, steroids, coumarins and glycosides in the plant extracts with each plant possessing at least 3 classes of the antimalarial compounds. Organic extracts of V. capensis, N. costata and D. eriocarpum showed antiplasmodial activity at concentrations ranging from 50-250 ìg/mL. Extracts from D. eriocarpum showed the highest activity with an IC50 of 63.17ìg/mL followed by V. capensis and N. costata at 93.29ìg/mL and 86.63ìg/mL, respectively. All the plant extracts inhibited haemazoin accumulation with D. eriocarpum exhibiting the highest inhibition. The extracts also inhibited protease activity at the early ring stage where infection of red blood cells was being established and at the trophozoite stage where metabolism of the parasites was increased. These results support the ethno-medicinal uses for these plants as complementary medicine for malaria en_US
dc.format.extent xxi, 171 p en_US
dc.language.iso eng en_US
dc.source.uri en_US
dc.source.uri en_US
dc.subject Antimalarials en_US
dc.subject Medicinal plants en_US
dc.subject Malaria prevention en_US
dc.title Evaluation of antimalarial properties of indigenous plants used by traditional healers in Namibia en_US
dc.type Thesis en_US
dc.identifier.isis F004-199299999999999 en_US
dc.description.degree Windhoek en_US
dc.description.degree Namibia en_US
dc.description.degree University of Namibia en_US
dc.description.degree Master of Science en_US
dc.description.status en_US
dc.masterFileNumber 3837 en_US

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